Recombinant Mouse Interleukin-4 (IL-4 Mouse)
Product Data Sheet and Certificate of Analysis
Synonyms: BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Introduction: Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells. Multiple immune response-modulating functions are performed by IL-4 on a variety of cell types and it has an important role in the regulator of isotype switching, induction of IgE production in B lymphocytes and differentiation of precursor T helper cells. IL-4 binds to both membrane-bound and secreted soluble IL-4 receptors.
Description: Interleukin-4 mouse recombinant protein produced in E. coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13.5kDa.
Source: Escherichia coli.
Physical Appearance: Sterile filtered white lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Solubility: It is recommended to reconstitute the lyophilized Interleukin 4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized interleukin-4 stable at room temperature for a week, however should be stored below -20°C. Upon reconstitution IL4 should be stored below -20°C for future use. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid multiple freeze-thaw cycles.
Purity and Protein content: Greater than 95.0% as determined by
- UV spectroscopy at 280nm
- Analysis by HPLC
- Quantitation by PAGE under reducing and non-reducing conditions
Endotoxin Level: Endotoxin levels, as measured by LAL analysis is <1.0EU/µg of protein.
Amino Acid Sequence:
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S.
Biological Activity: The biological activity determined by the dose-dependent proliferation of murine HT-2 cells is between than 1.08-3.8ng/ml.
Usage: Unless otherwise mentioned in the product description this product is intended for LABORATORY RESEARCH USE ONLY. NOT FOR HUMAN USE.