Recombinant Human Transforming Growth Factor-beta 1 (TGF-beta1 Human); 10ug

Description

Recombinant Human Transforming Growth Factor-beta 1, (TGF-beta1 Human); 10ug

Product Data Sheet and Certificate of Analysis

Synonyms: Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.

Introduction: Transforming growth factor beta (TGF-beta) mediates many cell-cell interactions that occur during embryonic development. Three TGF-betas have been identified in mammals. TGF-beta1, TGF-beta2 and TGF-beta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.

Description: TGFb1 Human Recombinant produced in Human 293 cells is a homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25kDa. The TGFb1 is purified by proprietary chromatographic techniques.

Source: Human 293 cells.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized from a sterile filtered solution containing 0.1 % trifluoroacetic acid (TFA) And trehalose (1:20 protein to Trehalose ratio).

Solubility: It is recommended to reconstitute the lyophilized TGFbeta1 in sterile 5mM HCl & 50ug/ml BSA at a concentration of 0.1mg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized TGFb1 although stable at room temperature for 3 weeks, should be stored desiccated below -20°C. Upon reconstitution TGFb1 Human should be stored at below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent multiple freeze-thaw cycles.

Purity: Greater than 98.0% as determined by SDS-PAGE.

Amino acid sequence:

ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS.

Biological Activity: The ED50, as measured by the dose-dependent inhibition of IL-4-induced proliferation of mouse HT-2 cells, was found to be 0.126ng/ml.

Usage: Unless otherwise mentioned in the product description this product is intended for LABORATORY RESEARCH USE ONLY.