Recombinant Mouse Interleukin-4; 20ug

Description

Recombinant Mouse Interleukin-4; 20ug

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Synonyms: BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.

Introduction:  Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells. Multiple immune response-modulating functions are performed by IL-4 on a variety of cell types and it has an important role in the regulator of isotype switching, induction of IgE production in B lymphocytes and differentiation of precursor T helper cells. IL-4 binds to both membrane-bound and secreted soluble IL-4 receptors.

Description: Interleukin-4 Mouse Recombinant protein produced in E. coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13.5 kDa.

Source: Escherichia coli

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized from a concentrated (1mg/ml) solution in PBS pH 7.4.

Solubility: It is recommended to reconstitute the lyophilized Interleukin 4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Interleukin-4 stable for 3 weeks, should be stored desiccated below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please avoid multiple freeze-thaw cycles.

Purity: Greater than 96.0% as determined by: (a) Analysis by RP-HPLC and (b) SDS-PAGE

Amino acid sequence: MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S.

Biological Activity: The ED50 as determined by the dose-dependent proliferation of Murine HT-2 cells, corresponding to a Specific Activity of IUnits/mg.

Usage: Unless otherwise mentioned in the product description this product is intended for LABORATORY RESEARCH USE ONLY.