Recombinant Mouse Interleukin-2; 20ug

Description

Recombinant Mouse Interleukin-2; 20ug

Other Sizes are available please ask for Quote

Synonyms: Interleukin-2, T cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.

Description: Interleukin-2 Mouse Recombinant protein produced in E. coli is a single, non-glycosylated polypeptide chain containing 149 amino acids and having a molecular mass of 17.23 kDa.

Source: Escherichia coli

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein (1mg/ml) was lyophilized extensive dialysis against 10mM sodium citrate buffer to a pH=4.

Solubility: It is recommended to reconstitute the lyophilized Mouse IL-2 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -20°C. Upon reconstitution IL2 should be stored  at -20°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please avoid multiple freeze-thaw cycles.

Purity: Greater than 98.0% as determined by:

(a) Analysis by RP-HPLC and (b) SDS-PAGE

Amino acid sequence:

PTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ

Biological Activity: The ED₅₀ as determined by the dose-dependant stimulation of murine CTLL-2 cells is 0.2ng/ml corresponding to a Specific Activity of 5×10⁶IU/mg.

Usage: Unless otherwise mentioned in the product description this product is intended for LABORATORY RESEARCH USE ONLY.