Recombinant Human Interleukin-2; 50ug

Description

Recombinant Human Interleukin-2; 50ug

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Synonyms: T cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.

Description: Interleukin-2 human recombinant protein produced in E. coli is a single, non-glycosylated mutein (variant form) of human IL-2 polypeptide chain containing 134 amino acids and having a molecular mass of 15.517 kDa. Interleukin-2 has a Ser substitute for Cysteine at position 126. The IL-2 is purified by proprietary chromatographic techniques.

Source: Escherichia coli.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein (1.1mg/ml) was lyophilized after extensive dialysis against 0.17mg sodium monobasic and 0.89mg dibasic sodium phosphate buffer to a pH=7.5.

Solubility: It is recommended to reconstitute the lyophilized interleukin 2 in sterile 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Interleukin-2 stable at room temperature for one week, should be stored desiccated below -20⁰C. Upon reconstitution IL2 should be stored below -20⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent multiple freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: SDS-PAGE

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells in ng/ml, corresponding to a Specific Activity of IU/mg.

Usage: Unless otherwise mentioned in the description this product is intended for LABORATORY RESEARCH USE ONLY. NOT FOR HUMAN USE.