Recombinant Human GM-CSF; 20ug

Description

Recombinant Human  Granulocyte Macrophage Colony Stimulating Factor (GM-CSF); 20ug

Other Sizes are available please ask for Quote

Synonyms: CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897.

Introduction: GM-CSF is a cytokine that controls the production, differentiation and function of granulocytes and macrophages. The active form of protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.

GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Description: Granulocyte Macrophage Colony Stimulating Factor Human Recombinant protein produced in E. coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14.4 kDa.

GM-CSF is purified by proprietary chromatographic techniques.

Source: Escherichia coli.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: GM-CSF was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4±0.1.

Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -20°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent multiple freeze-thaw cycles.

Purity: Greater than 98.0% as determined by:

Analysis by RP-HPLC and SDS-PAGE.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. N-terminal methionine has been completely removed enzymatically.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1ng/ml, corresponding to a Specific Activity of 11.1×10⁶ IU/mg.

Protein content: GM-CSF quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1mg/ml) solution.
2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.

Usage: Unless otherwise mentioned in the product description this product is intended for the LABORATORY RESEARCH USE ONLY.