Recombinant Mouse Granulocyte Macrophage Colony Stimulating Factor; 20ug

Description

Recombinant Mouse Granulocyte Macrophage Colony Stimulating Factor; 20ug

Other Sizes are available please ask for Quote

Synonyms: CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim.

Description: Granulocyte Macrophage Colony Stimulating Factor Mouse Recombinant protein produced in E. coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14.28 kDa.

GM-CSF Mouse is purified by proprietary chromatographic techniques.

Source: Escherichia coli

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: GM-CSF Mouse was lyophilized with no additives.

Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored below -20°C. Upon reconstitution GM-CSF should be stored  below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please avoid multiple freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC and (b) SDS-PAGE

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cell line is <0.2 ng/ml, corresponding to a Specific Activity of  5×10⁶ IU/mg.

Usage: Unless otherwise mentioned in the product description this product is intended for LABORATORY RESEARCH USE ONLY.